thp1 atcc tib 202tm cell cultures  (ATCC)

Journal: Frontiers in Immunology

Article Title: STING activation by teniposide: a potential direct mechanism beyond cGAS stimulation

doi: 10.3389/fimmu.2025.1677836

Figure Lengend Snippet: Teniposide is a potential STING agonist that induces IFNB1 gene expression. (a) Schematic representation of high-throughput virtual screening HTVS of NIH libraries targeting the STING binding pocket. (b) Representation of the 2D structures of Teniposide and cGAMP. (c) Relative IFNB1 gene expression in THP1 cells treated with varying doses of Teniposide or cGAMP. (d) Time-course IFNB1 gene expression in THP1 cells treatment with cGAMP or Teniposide. (e) MX1 and IL6 gene expression in THP1 cells 12h post-Teniposide treatment. (f) Dose response of Teniposide in BMDMs, showing relative IFNB1 expression 24h post-treatment. (g) IFNB1 time-course expression in BMDM upon stimulation with Teniposide (1µM or 3µM). cGAMP induction at 8h was used as a reference control. (h) Relative MX1 and IL6 gene expression in BMDM 48h after Teniposide treatment. Statistical significance is indicated by *p ≤ 0.05, **p ≤ 0.01, ***p ≤0.005, **** p ≤ 0.001.

Article Snippet: Thp1 ATCC ® TIB-202TM cell cultures are human monocyte cells obtained from peripheral blood of patients with acute monocytic leukemia.

Techniques: Gene Expression, High Throughput Screening Assay, Binding Assay, Expressing, Control

Journal: Frontiers in Immunology

Article Title: STING activation by teniposide: a potential direct mechanism beyond cGAS stimulation

doi: 10.3389/fimmu.2025.1677836

Figure Lengend Snippet: Teniposide-induced IFNB1 expression requires STING and may occur independently of cGAS and IFI16. (a) IFNB1 relative gene induction measured by RT-qPCR in WT or STING KO THP1 cells after Teniposide 3µM treatment for 8h. (b) IFNB1 relative expression in WT or GT STING BMDM treated with 3µM Teniposide for 8h. (c) Western blot representing pTBK1, total TBK1, pIRF3, total IRF3 and bacting in THP1cells mock treated, treated for 4h with 3µM of Teniposide or 1µM of diABZI. (d) IFNB1 relative gene expression in WT THP1 cells stimulated for 18h with 10 IU of IFN-β/ml or mock-treated followed by a treatment with DMSO, cGAMP or Teniposide (3µM). (e) Same as in (d) but in WT and cGAS KO THP1 cells. (f) Same as in (d) but in WT and IFI16 THP1 KO cells. (g) Western blot verifying STING, cGAS or IFI16 in WT but not STING, cGAS, and IFI16 KO THP1 cells. Tubulin or β-Actin is shown as a loading control. Statistical significance is indicated by *p ≤ 0.05, **p ≤ 0.01, ***p ≤0.005, **** p ≤ 0.001.

Article Snippet: Thp1 ATCC ® TIB-202TM cell cultures are human monocyte cells obtained from peripheral blood of patients with acute monocytic leukemia.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Gene Expression, Control